2. Product
  3. Target Validation
  4. ID3EAL Assay Search
Assay Search
  • Assay Principle

    MiRXES ID3EAL miRNA qPCR solution adopts a 3-specific assay priming strategy. The conformational-restricted miRNA-specific RT primer efficiently hybridizes to the mature but not the precursor form of the target miRNA. Specific qPCR forward and reverse primers confer further amplification specificity. Coupled with high performance proprietary ID3EAL RT and qPCR master mixes, robust amplification can be completed in just 2 hours with enhanced signal-to-noise ratio.

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  • Fast and Simple Workflow

    MiRXES ID3EAL miRNA Assay workflow illustration.
    Fast and simple workflow to get from RNA-to-Ct in just 2 hours, allowing for fast turnaround and improved throughput.
    Allows multiplexing of the reverse transcription step (up to 10 targets) to enhance the assay specificity

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  • Superior Sensitivity

    MiRXES ID3EAL miRNA qPCR Solution is designed to offer superior sensitivity, a broad dynamic range, and high amplification efficiencies consistently even in difficult samples such as plasma.

    Single base differences frequently occur, especially in miRNAs that are closely related, and poses a common challenge in miRNA quantification. Utilizing the unique 3-specific primers strategy, MiRXES ID3EAL miRNA qPCR Solution enables discrimination of highly homologous miRNA family members with single nucleotide difference.

    Comparison of Ct values obtained for five miRNA targets with a range of AT contents across four different vendors. MiRXES ID3EAL miRNA PCR Solution showed the highest performance with significantly earlier Ct values emerging for all miRNA targets tested as compared to other vendors. The proprietary MiRXES ID3EAL Assays Algorithm allows the design of robust assays that work with difficult miRNA targets giving users the confidence in their profiling data.

    MiRXES ID3EAL miRNA qPCR Solution detected >450 miRNAs whereas less than 100 miRNAs were reliably detected by other platforms in equivalent volume of serum samples (i.e. 200μL).
  • Superior Specificity

    Single base differences frequently occur, especially in miRNAs that are closely related, and poses a common challenge in miRNA quantification. Utilizing the unique 3-specific primers strategy, MiRXES ID3EAL miRNA qPCR Solution enables discrimination of highly homologous miRNA family members with single nucleotide difference.

    Single Nucleotide Discrimination Test. Synthetic cDNAs, representing the various isoforms of the highly homologous let-7 miRNA family, were used as templates to challenge assay specificity. The percentage relative detection was calculated from the Ct values collected from matched and mismatched assays. Using MiRXES proprietary thermodynamics based algorithms, MiRXES ID3EAL microRNA qPCR Solution was able to deliver specificity down to a single base difference, with little to no assay cross-reactivity. Sequences of the targets are shown on the left with base changes highlighted in orange and green.