Unique RT Primer:
Conformational restricted miRNA specific RT primer efficiently hybridizes to mature but not precursor form of target miRNA.
Specific Real-Time PCR Primers:
miRNA specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification of amplicon.
Tailored RT-qPCR Reagents:
Optimized RT and qPCR master mixes enhance signal to noise ratio.
Optimized RT-qPCR primers and reagents to drive efficient target amplification from limiting amounts (≥1pg) of input RNA sample.
No universal primers. Every assay utilizes three miRNA specific primers to discriminate single nucleotide differences.
RNA to Ct in less than 2 hours for faster turnaround and improved throughput.
Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.
Complete kit to minimize set-up time. Compatible with all major qPCR instruments.
The unique three primer design of the ID3EAL miRNA qPCR Assays yields class leading sensitivity compared to other miRNA detection systems. These assays are shown to have more consistent performance over miRNAs with varying sequences especially those with high AT content
Single base differences frequently occur, especially in miRNAs that are closely related, and poses a common challenge in miRNA quantification. Utilizing the unique 3-specific primers strategy, MiRXES ID3EAL miRNA qPCR Solution enables discrimination of highly homologous miRNA family members with single nucleotide difference.
MiRXES ID3EAL miRNA qPCR assays yielded highly reproducible results in profiling more than 200 miRNAs from 30 cancer sera over a year in two independent laboratories (R2> 0.95). The ease of use enables even first time users to generate consistent technical and biological replicates.